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Real-Time Nucleic Acid Sequence-Based Amplification in Nanoliter Volumes

✍ Scribed by Gulliksen, Anja; Solli, Lars; Karlsen, Frank; Rogne, Henrik; Hovig, Eivind; Nordstrøm, Trine; Sirevåg, Reidun


Book ID
121302815
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
347 KB
Volume
76
Category
Article
ISSN
0003-2700

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Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit realt