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Real time monitoring of junction ribonuclease activity of RNase H using chimeric molecular beacons

โœ Scribed by Liu, Bin; Xiang, Dan; Long, Ying; Tong, Chunyi


Book ID
121502249
Publisher
Royal Society of Chemistry
Year
2013
Tongue
English
Weight
556 KB
Volume
138
Category
Article
ISSN
0003-2654

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t