Real-Time Monitoring in Vitro Transcript
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Jianwei Liu; Patricia Feldman; Thomas D.Y. Chung
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Article
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2002
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Elsevier Science
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English
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A homogeneous fluorescence-based molecular beacon (MB) method has been developed for real-time monitoring of in vitro transcription reactions. MB probes are structured as target-specific antisense oligodeoxynucleotides containing a proximate fluorophore-quencher pair. Upon binding to its target sequ