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Real-time monitoring of double-stranded DNA cleavage using molecular beacons

โœ Scribed by Changbei Ma; Zhiwen Tang; Xiqin Huo; Xiaohai Yang; Wei Li; Weihong Tan


Book ID
116903042
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
305 KB
Volume
76
Category
Article
ISSN
0039-9140

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Real-Time Monitoring in Vitro Transcript
โœ Jianwei Liu; Patricia Feldman; Thomas D.Y. Chung ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 108 KB

A homogeneous fluorescence-based molecular beacon (MB) method has been developed for real-time monitoring of in vitro transcription reactions. MB probes are structured as target-specific antisense oligodeoxynucleotides containing a proximate fluorophore-quencher pair. Upon binding to its target sequ