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Real-time electrochemical monitoring of isothermal helicase-dependent amplification of nucleic acids

✍ Scribed by Kivlehan, Francine; Mavré, François; Talini, Luc; Limoges, Benoît; Marchal, Damien

Book ID
121338367
Publisher
Royal Society of Chemistry
Year
2011
Tongue
English
Weight
508 KB
Volume
136
Category
Article
ISSN
0003-2654

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Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit realt