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Real-time Detection of Nucleotide Incorporation During Complementary DNA Strand Synthesis

✍ Scribed by Alexander Krieg; Stephan Laib; Thomas Ruckstuhl; Stefan Seeger


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
79 KB
Volume
4
Category
Article
ISSN
1439-4227

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Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit realt