Real-Time, Sequence-Specific Detection o
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James G. Nadeau; J.Bruce Pitner; C.Preston Linn; James L. Schram; Cheryl H. Dean
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Article
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1999
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Elsevier Science
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English
β 140 KB
Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit realt