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Reaktive Träger auf der Basis von Polyethylenimin zur Immobilisierung von Enzymen

✍ Scribed by Manecke, Georg ;Heydolph, Sabine

Publisher
Wiley (John Wiley & Sons)
Year
1981
Weight
790 KB
Volume
182
Category
Article
ISSN
0025-116X

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✦ Synopsis

Abstract

Reactive slightly basic carriers with isothiocyanato and azido groups, which are able to bind enzymes covalently, could be obtained by reaction of crosslinked and uncrosslinked poly‐(ethyleneimine) with 4‐nitrophenyl isothiocyanate, 4‐nitrophenyl isocyanate, or ethyl chloroacetate, respectively, and subsequent reactions. The binding ability of the carriers for papain, trypsin, α‐chymotrypsin, and urease in dependence of the content of reactive groups and in the case of urease as function of the amount of offered enzyme was investigated. To characterize the immobilized enzymes some properties as enzymatic activity, storage stability, and stability after repeated application were determined.

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