Reaktive copolymere des N-vinyl-2-pyrrolidons zur immobilisierung von enzymen
✍ Scribed by Manecke, Georg ;Korenzecher, Rafael
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1977
- Weight
- 516 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0025-116X
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✦ Synopsis
Abstract
1‐Vinyl‐2‐pyrrolidone was copolymerized with maleic acid anhydride, with acrylic acid, and with tyrene respectively, using 1,4‐divinylbenzene as crosslinking agent. By polymeranalogous reactions isothiocyanato‐ and diazo groups were introduced into the styrene copolymers, respectively, and azido groups into the acrylic acid copolymers. These reactive, insoluble, hydrophilic carriers were used for the immobilization of enzymes by covalent binding. The copolymers, particularly their ability to immobilize papain as well as their enzymatic activity, were investigated.
📜 SIMILAR VOLUMES
The polymerization rate of the ternary monomer system, styrene, N-vinyl-2-pyrrolidone, and methyl methacrylate, was investigated in bulk at 60 "C with AIBN as initiator. The results were interpreted by means of the well known classical reaction model and a newly derived simplified reaction model. Th