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Reaction of vitamin k and dithiothreitol on reversed-phase C18 high-performance liquid chromatographic columns

โœ Scribed by Louise M. Canfield; Rita B. Holzman

Book ID
104146759
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
524 KB
Volume
299
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

A non-enzymatically catalyzed side reaction between dithiothreitol and vitamin K occurs on reversed-phase C1 s high-performance liquid chromatographic columns. Excess dithiothreitol may be effectively removed from enzymatic reaction mixtures with N-ethylmaleimide. A low polarity gradient elution system is reported which gives quantitative recoveries (greater than 95%) of the vitamins in a concentration range useful for analysis of enzymatically catalyzed reactions.

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