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High-performance liquid chromatographic analysis of chrysin derivatives on a Nova-Pak® C18column

✍ Scribed by Kyoung Soon Kim; Joon Su Shin; Youmie Park; Sanghyun Lee; Yang Bae Kim; Bak-Kwang Kim

Book ID
105672745
Publisher
Springer
Year
2002
Tongue
English
Weight
232 KB
Volume
25
Category
Article
ISSN
0253-6269

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A non-enzymatically catalyzed side reaction between dithiothreitol and vitamin K occurs on reversed-phase C1 s high-performance liquid chromatographic columns. Excess dithiothreitol may be effectively removed from enzymatic reaction mixtures with N-ethylmaleimide. A low polarity gradient elution sys