✦ LIBER ✦

Reaction of histidine residues in proteins with diethylpyrocarbonate: Differentlal molar absorptivities and reactivities

✍ Scribed by Joseph L. Roosemont

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 362 KB
Volume: 88
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90424-4

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✦ Synopsis

The applicability of the spectrophotometric determination of histidine according to Ovadi et al. [(1967) Acta Biochim. Biophys. Acad. Sci. Hung. 2, 455-4581 to a large range of molar ratios of diethylpyrocarbonate (DEP) to histidine requires the use of appropriate differential molar absorptivities dependent on the DEP concentration used. This improved procedure allows a more differentiated aproach to the reactivity of histidine residues in proteins.

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