𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Reaction of 7,12-dimethylbenz(a)anthracene with DNA of fetal and maternal rat tissues in vivo

✍ Scribed by Gerhard Doerjer; Helga Diessner; Joachim Bücheler; Paul Kleihues

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
374 KB
Volume
22
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Pregnant BD‐IX rats (21st day of gestation) received a single IV injection (15 mg/kg) of tritiated 7,12‐dimethylbenz(a)anthracene (DMBA), a dose known to induce a high incidence of nervous‐system tumors in the offspring. The animals were killed 12 h later and hydrocarbon‐deoxyribonucleoside products from DNA of maternal and fetal tissues were separated on Sephadex LH‐20 columns eluted with a 20–100% methanol gradient. Concentrations of the major DMBA‐DNA adduct varied considerably, with highest values in maternal intestine, liver and lung, followed by spleen, kidney and brain. In fetal intestine and liver, concentrations were 34% and 16% lower than in the respective maternal organs whereas the reaction with cerebral DNA was 2 1/2 times higher in fetuses than in the pregnant mother. This indicates that there is no significant placental barrier to DMBA or DMBA metabolites involved in DNA binding and that rat fetuses participate in the metabolic formation of the ultimate carcinogen.

📜 SIMILAR VOLUMES

DNA sequence analysis of hprt mutations
DNA sequence analysis of hprt mutations in lymphocytes from Sprague-Dawley rats treated with 7,12-dimethylbenz[a]anthracene
✍ Robert H. Heflich; Roberta A. Mittelstaedt; Mugimane G. Manjanatha; Lascelles E. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 799 KB

Treatment of female SpragueDawley rats with the potent mammary gland carcinogen 7,12dimethyC benz[a]anthracene (DMBA) results in the formation of DNA adducts with dG and dA and in the induction of 6thioguanineresistant (TG') lymphocyte mutants. In this study, we have examined the types of mutations

Tumorigenesis of mammary gland by 7,12 d
Tumorigenesis of mammary gland by 7,12 dimethylbenz(a)anthracene during pregnancy: Relationship with DNA synthesis
✍ Dilip K. Sinha; James E. Pazik 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 French ⚖ 389 KB

## Abstract The relationship between mammary cell proliferation during pregnancy and susceptibility to 7,12‐dimethyl‐benz(a)anthracene (DMBA) was examined. DMBA was administered intravenously to Sprague‐Dawley rats on the 5th, 10th or 15th day of pregnancy. [^3^H]thymidine labelling index (LI) of t

Gene expression profiling in the mammary
Gene expression profiling in the mammary gland of rats treated with 7,12-dimethylbenz[a]anthracene
✍ Andriana D. Papaconstantinou; Ilanchezhian Shanmugam; Liang Shan; Insa S. Schroe 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 875 KB

## Abstract Identification of molecular markers of early‐stage breast cancer development is important for the diagnosis and prevention of the disease. In the present study, we used microarray analysis to examine the differential expression of genes in the rat mammary gland soon after treatment with

Deregulated expression of the PCPH proto
Deregulated expression of the PCPH proto-oncogene in rat mammary tumors induced with 7,12-dimethylbenz[a]anthracene
✍ Montserrat Solanas; Eduard Escrich; Ana Rouzaut; Irmgard Costa; Alfredo Martínez 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 422 KB

## Abstract The __PCPH__ proto‐oncogene was identified by its frequent activation in Syrian hamster fetal cells exposed to 3‐methylcholanthrene. We previously isolated human __PCPH__ cDNA and studied its expression in normal human tissues. We report herein the pattern of __PCPH__ expression in norm

Loss of heterozygosity at the N-ras locu
Loss of heterozygosity at the N-ras locus in 7,12-dimethylbenz[a]anthracene-induced rat leukemia
✍ Mitsuhiko Osaka; Shinji Matsuo; Takashi Koh; Taketoshi Sugiyama 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 133 KB 👁 2 views

The 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat leukemia model enables scientists to analyze cells altered by carcinogens at various stages of leukemogenesis. We have reported that a consistent type of point mutation, A→T transversion at the second base in codon 61 of the N-ras gene, was prese