A class of F' plasmids, designated Fpoh+, was previously shown to be able to replicate extra-chromosomally on Hfr strains by virtue of carrying the specific site or region poh+ (permissive on Hfr) of the E. coli chromosome (Hiraga, 1975, 1976a). These plasmids were now found to replicate on E. coli
Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli
β Scribed by Van Brunt, Jennifer ;Waggoner, Barbara T. ;Pato, Martin L.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 684 KB
- Volume
- 150
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
The replication of an F' plasmid in a dnaC mutant, thermolabile for initiation of chromosomal replication, has been re-examined using a novel DNA-DNA annealing assay. Plasmid replication ceases rapidly at non-permissive conditions, consistent with a direct role for the dnaC product in the replication of F.
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