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Chromosome replication pattern in dam mutants of Escherichia coli

✍ Scribed by Louarn, Jacqueline ;Fran�ois, Vincent ;Louarn, Jean-Michel

Publisher
Springer
Year
1990
Tongue
English
Weight
382 KB
Volume
221
Category
Article
ISSN
0026-8925

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F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30-200 times higher than the isogenic dam+ strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduct

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Host capacity for growth of single-stranded DNA phages was investigated with several replication mutants of E. coli. In dnaL708, dnam709 and dnaS707 mutants, multiplication of phiK was not restricted even at 42 degree C. In dnaM710 cells, however, growth of phiK was severely effected at 42 degree C