๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Rational Design of Fluorescent Biosensor for Cyclic di-GMP

โœ Scribed by Chun Loong Ho; Siew Lee Koh; Mary Lay Cheng Chuah; Zhen Luo; Wei Jian Tan; Donovan Kian Soon Low; Prof. Dr. Zhao-Xun Liang

Book ID
102791851
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
648 KB
Volume
12
Category
Article
ISSN
1439-4227

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A continuous fluorescence assay for cycl
A continuous fluorescence assay for cyclic nucleotide phosphodiesterase hydrolysis of cyclic GMP
โœ J.David Johnson; John D. Walters; John S. Mills ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 376 KB

The fluorescent 2'-methylanthraniloyl derivative of cyclic GMP undergoes a 45% decrease in fluorescence when it is cleaved by brain phosphodiesterase in the presence of calmodulin. This fluorescence decrease is dependent upon calcium, calmodulin, and phosphodiesterase, and correlates well (r = 0.996