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Rates of male pronuclear enlargement in sea urchin zygotes

โœ Scribed by Luttmer, Shirley J. ;Longo, Frank J.

Book ID
102894869
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
844 KB
Volume
243
Category
Article
ISSN
0022-104X

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โœฆ Synopsis

Rates of male pronuclear enlargement were determined using synchronous populations of fertilized sea urchin eggs (Arbacia punctulata and Lytechinus variegatus), fixed at regular intervals following insemination and stained with Hoechst 33342. The rate of male pronuclear expansion in both Arbacia and Lytechinus zygotes was linear and significantly affected by polyspermy, temperature, metabolic inhibitors (dinitrophenol, potassium cyanide, sodium azide, antimycin A, and oligomycin), and blockage of cytoplasmic alkalinization. Inhibitors of protein synthesis (emetine and puromycin) and disrupters of cytoskeletal elements (colchicine, nocodazole and cytochalasin B) had no effect on the rate of male pronuclear enlargement.

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โœ Maria Imschenetzky; Marcia Puchi; Cecilia Pimentel; Alejandra Bustos; Margarita ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 935 KB

To obtain information on the remodeling of sperm chromatin during male pronuclei formation, we have followed the sperm specific histones (SpH) that form the nucleosomal core by Western imunoblot analysis with policlonal antibodies directed against the core SpH. The results obtained indicate that the