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In Vitro Development of the Sea Urchin Male Pronucleus

โœ Scribed by L.A. Cameron; D.L. Poccia

Book ID
115597147
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
912 KB
Volume
162
Category
Article
ISSN
0012-1606

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๐Ÿ“œ SIMILAR VOLUMES

Formation of the sea urchin male pronucl
Formation of the sea urchin male pronucleus in cell-free extracts
โœ P. Collas ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 128 KB

At fertilization, the dormant sperm nucleus undergoes morphological and biochemical transformations leading to the development of a functional male pronucleus. We have investigated the formation of the male pronucleus in a cell-free system consisting of permeabilized sea urchin sperm nuclei incubate

Formation of the sea urchin male pronucl
Formation of the sea urchin male pronucleus in vitro: Membrane-independent chromatin decondensation and nuclear envelope-dependent nuclear swelling
โœ Philippe Collas; Dominic L. Poccia ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 895 KB

## Abstract We demonstrate that complete sea urchin male pronuclear development in vitro is a twoโ€step process involving membraneโ€independent chromatin decondensation and nuclear envelopeโ€dependent pronuclear swelling. In the absence of cytoplasmic membrane vesicles (MVs), permeabilized sperm chrom

Immunobiochemical evidence for the loss
Immunobiochemical evidence for the loss of sperm specific histones during male pronucleus formation in monospermic zygotes of sea urchins
โœ Maria Imschenetzky; Marcia Puchi; Cecilia Pimentel; Alejandra Bustos; Margarita ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 935 KB

To obtain information on the remodeling of sperm chromatin during male pronuclei formation, we have followed the sperm specific histones (SpH) that form the nucleosomal core by Western imunoblot analysis with policlonal antibodies directed against the core SpH. The results obtained indicate that the

Identification of a cysteine protease re
Identification of a cysteine protease responsible for degradation of sperm histones during male pronucleus remodeling in sea urchins
โœ Maria Imschenetzky; Fredy Dรญaz; Martin Montecino; Felipe Sierra; Marcia Puchi ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 379 KB

We have identified a 60-kDa cysteine protease that is associated with chromatin in sea urchin zygotes. This enzyme was found to be present as a proenzyme in unfertilized eggs and was activated shortly after fertilization. At a pH of 7.8-8.0, found after fertilization, the enzyme degraded the five sp