At fertilization, the dormant sperm nucleus undergoes morphological and biochemical transformations leading to the development of a functional male pronucleus. We have investigated the formation of the male pronucleus in a cell-free system consisting of permeabilized sea urchin sperm nuclei incubate
Two Steps Required for Male Pronucleus Formation in the Sea Urchin Egg
β Scribed by C.C. Cothren; D.L. Poccia
- Book ID
- 115601411
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 648 KB
- Volume
- 205
- Category
- Article
- ISSN
- 0014-4827
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To obtain information on the remodeling of sperm chromatin during male pronuclei formation, we have followed the sperm specific histones (SpH) that form the nucleosomal core by Western imunoblot analysis with policlonal antibodies directed against the core SpH. The results obtained indicate that the
## Abstract We demonstrate that complete sea urchin male pronuclear development in vitro is a twoβstep process involving membraneβindependent chromatin decondensation and nuclear envelopeβdependent pronuclear swelling. In the absence of cytoplasmic membrane vesicles (MVs), permeabilized sperm chrom