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Rates of incorporation of radioactive molecules during the cell cycle

✍ Scribed by J. W. Gray; M. G. Pallavicini; Y. S. George; V. Groppi; M. Look; P. N. Dean

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
763 KB
Volume
108
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

We report measurements of the incorporation of radioactive molecules during short labeling periods, as a function of cell‐cycle stage, using a cell‐sorter‐based technique that does not require cell synchronization. We have determined: (1) tritiated thymidine (^3^H‐TdR) incorporation throughout S‐phase in Lewis lung tumor cells in vitro both before and after treatment with cytosine arabinoside; (2) ^3^H‐TdR incorporation throughout S‐phase in KHT tumor cells in vitro and in vivo; (3) ^3^H‐TdR incorporation throughout S‐phase in Chinese hamster ovary cells and compared it with DNA synthesis throughout S‐phase; (4) a mathematical expression describing ^3^H‐TdR incorporation throughout S‐phase in Chinese hamster M3‐1 cells; and (5) the simultaneous incorporation of ^3^H‐TdR and ^35^S‐methionine as they are related to cell size and DNA content in S49 mouse lymphoma cells. In asynchronously growing cells in vitro and in vivo, ^3^HH‐TdR incorporation was generally low in early and late S‐phase and highest in mid‐S‐phase. However, in Lewis lung tumor cells treated with cytosine arabinoside ^3^H‐TdR incorporation was highest in early and late S‐phase and lowest in mid‐S‐phase. Incorporation of ^35^S‐methionine increased continuously with cell size and DNA content. Incorporation of ^3^H‐TdR in CHO cells was proportional to DNA synthesis.

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