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Modulation in the rates of incorporation of lipid precursors during the cell cycle

✍ Scribed by C. A. Lingwood; D. B. Thomas

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 365 KB
Volume: 86
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040860508

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✦ Synopsis

The rates of incorporation of 3H-choline into phospholipid and of 3H-fucose into glycolipid have been measured during the cell cycle of the murine mastocytoma, P815Y, synchronized by either velocity sedimentation or excess thymidine blockade. The rate of 3H-choline incorporation into acid-insoluble material exhibited two distinct maxima coincident with the early S and G2 periods, whereas the rate of incorporation of 3H-fucose into lipid extractable material was maximal during the G2 period. Variation in rate of incorporation of 3H-choline could not be accounted for by changes in membrane permeability.

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