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Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

✍ Scribed by Casey, James; Davidson, Norman


Book ID
121384344
Publisher
Oxford University Press
Year
1977
Tongue
English
Weight
596 KB
Volume
4
Category
Article
ISSN
0305-1048

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Kinetics and Thermodynamics of DNA, RNA,
✍ Rauzan, Brittany; McMichael, Elizabeth; Cave, Rachel; Sevcik, Lesley R.; Ostrosk 📂 Article 📅 2013 🏛 American Chemical Society 🌐 English ⚖ 541 KB

The rates of duplex formation for two octamers of DNA (5′ d-CACGGCTC/5′ d-GAGCCGTG and 5′ d-CACAGCAC/5′ d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, ΔH°and ΔS°, have been de