Purification of rat liver lysosomal α-gl
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C. Dissous; J.F. Ansart; A. Cheron; J. Krembel
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Article
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1981
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Elsevier Science
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English
⚖ 571 KB
## Lysosomal u-glucosidase (EC 3.2.1.20) was purified from a lysosome-enriched fraction of rat liver using an improved procedure. A purification factor of 2900-fold was reached, with a yield of 35%. Polyacrylamide gel electrophoresis of the purified enzyme in nondenaturing conditions, or in the p