Rapid structural analysis of the in vitro and in vivo metabolism of SK&F 95448 by the combined use of thermospray liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry

✍ Scribed by Timothy J. A. Blake; Iain G. Beattie

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 558 KB
• Volume: 18
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200180822

✦ Synopsis

The thermospray liquid chromatography/mass spectrometry (LC/MS) interface has had a major impact on the direct analysis of the metabolic fate of xenobiotics in complex biological media. This paper outiines the rapidity and power of the LC/MS approach, and shows how detailed structural information can be obtained without recourse to individual compound isolation. This provides a great saving in time and effort. The additional specificity of liquid chromatography/tandem mass spectrometry is highlighted in identifying the sites of metaboiic transformation. The ability to handie biological sampies with littie or no clean-up usiog wide higb-performance liquid chromatographic gradients is a key feature of the success of this methodology.