Rapid separation of flavonoids by analytical high-speed counter-current chromatography

✍ Scribed by Tian-You Zhang; Rong Xiao; Zhuo-Ying Xiao; Lewis K. Pannell; Yoichiro Ito

• Book ID: 104145728
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 502 KB
• Volume: 445
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84521-0

✦ Synopsis

A commercial model of the analytical high-speed counter-current chromatography instrument was used for separation of flavonoids from a crude ethanol extract of dried fruits of sea buckthorn (Hippophae rhamnoides). Using a two-phase solvent system of chloroform-methanol-water (4:3:2), a five-fold increase in flow-rate of the mobile phase from 60 to 300 ml/h resulted in a rapid separation of five components in less than 15 min without significant loss in peak resolution. Major flavonoid component, isorhamnetin, was identified in its pure state by mass spectrometric analysis.