𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation of alkaloids from herbs using high-speed counter-current chromatography

✍ Scribed by Lei Fang; Yuqin Liu; Bin Yang; Xiao Wang; Luqi Huang

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
475 KB
Volume
34
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Alkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques. High‐speed counter‐current chromatography (HSCCC) is a liquid–liquid partition chromatography with a support‐free liquid stationary phase, and is gaining more and more popularity as a viable separation technique for bioactive compounds from natural resources. In the present review, focus is placed on the separation of alkaloids by both conventional HSCCC and pH‐zone‐refining counter‐current chromatography (CCC) techniques from herbs. The review presents the separation of over 120 different alkaloid compounds from more than 30 plant species by the conventional HSCCC and pH‐zone‐refining CCC. Based on the data from the literature, the proper solvent systems for the separation of alkaloids by the conventional HSCCC and pH‐zone‐refining CCC are also summarized.

📜 SIMILAR VOLUMES

Preparative separation of minor saponins
Preparative separation of minor saponins from Platycodi Radix by high-speed counter-current chromatography
✍ In J. Ha; Minseok Kang; Yun C. Na; Youmie Park; Yeong S. Kim 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 246 KB

## Abstract Platycosides (PSs), the saponins found in the root of __Platycodon grandiflorum__ (Jacq.) A. DC. (Platycodi Radix), are typically composed of oleanene backbones with two side chains; one is a 3‐__O__‐glucose linked by a glycosidic bond, and the other is a 28‐__O__‐arabinose‐rhamnose‐xyl

Preparative isolation of alkaloids from
Preparative isolation of alkaloids from Corydalis bungeana Turcz. by high-speed counter-current chromatography using stepwise elution
✍ Lili Niu; Zhensheng Xie; Tanxi Cai; Peng Wu; Peng Xue; Xiulan Chen; Zhiyong Wu; 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 436 KB

## Abstract High‐speed counter‐current chromatography (HSCCC) was successfully applied for the preparative separation and purification of alkaloids from __Corydalis bungeana__ Turcz. (Kudiding in Chinese) for the first time. After the measurement of partition coefficient of seven target alkaloids i

Preparative separation and purification
Preparative separation and purification of bufadienolides from Chinese traditional medicine of ChanSu using high-speed counter-current chromatography
✍ Jingkui Li; Xiaochi Ma; Fengyun Li; Jingkui Wang; Huirong Chen; Gang Wang; Xia L 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB

## Abstract A preparative high‐speed counter‐current chromatography method for isolation and purification of bufadienolides from ChanSu was developed by using a stepwise elution with two‐phase solvent system composed of __n__‐hexane/ethyl acetate/methanol/water at the ratios of 4:6:2:4 v/v, 4:6:2.5

Single-step separation of bioactive flav
Single-step separation of bioactive flavonol glucosides from Osteomeles schwerinae by high-speed counter-current chromatography
✍ Jun Lee; Dae Sik Jang; Nam Hee Yoo; Yun Mi Lee; Joo-Hwan Kim; Jin Sook Kim 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 154 KB

## Abstract Two bioactive flavonol glucosides, hyperoside and quercitrin, were successfully isolated in one step from the phytochemically unknown medicinal plant __Osteomeles schwerinae__ by high‐speed counter‐current chromatography using a two‐phase solvent system composed of __n__‐hexane–ethyl ac

Semi-preparative high-speed counter-curr
Semi-preparative high-speed counter-current chromatography separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn by pH-gradient elution
✍ Qingping Duanmu; Aifeng Li; Ailing Sun; Renmin Liu; Xiaopeng Li 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 195 KB

## Abstract A new high‐speed counter‐current chromatography method for semi‐preparative separation and purification of alkaloids from embryo of the seed of __Nelumbo nucifera__ Gaertn was developed by using pH‐gradient elution mode. Diethyl ether was used as the stationary phase of the two‐phase so

Droplet counter-current chromatography o
Droplet counter-current chromatography of indole alkaloids from Tabernaemontana hilariana
✍ Claudia Andrea Lima Cardoso; Dr. Wagner Vilegas 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 44 KB 👁 1 views

This paper reports the separation of the indole alkaloids from the benzene extract of the root barks of Tabernaemontana hilariana (Apocynaceae). The crude alkaloid fraction was fractionated by droplet counter-current chromatography using a low polarity mixture (hexane:ethyl acetate:ethanol:water). N