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Rapid purification of D-ribulose 1,5-bisphosphate carboxylase by vertical sedimentation in a reoriented gradient

✍ Scribed by Berhow, M.A.; Saluja, A.; McFardden, B.A.

Book ID
123135832
Publisher
Elsevier Science
Year
1982
Weight
486 KB
Volume
27
Category
Article
ISSN
0304-4211

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In a preparation of soluble components from isolated spinach (Spinecia oleracea L.) chloroplasts, the activity of ribulose-l,5-bisphosphate carboxylase (EC 4.1.1.39) is strongly increased by 6-phosphogluconate or by NADPH at pH 8.0. When the thylakoid system is added to these soluble components (rec