d-Ribulose-1,5-bisphosphate carboxylase-
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G.H. Lorimer; M.R. Badger; T.J. Andrews
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Article
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1977
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Elsevier Science
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English
⚖ 580 KB
Improved methods for the activation and assay of D-ribulose-1,5-bisphosphate carboxylase and oxygenase are described. The importance of fully activating the enzyme before starting either reaction is emphasized. The enzyme is activated by preincubation with 20 mM M&l, and 10 mM NaHCO,, pH 8.6. To avo