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Description and applications of a rapid and sensitive non-radioactive microplate-based assay for maximum and initial activity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase

✍ Scribed by RONAN SULPICE; HENDRIK TSCHOEP; MARIA VON KORFF; DIRK BÜSSIS; BJÖRN USADEL; MELANIE HÖHNE; HANNA WITUCKA-WALL; THOMAS ALTMANN; MARK STITT; YVES GIBON


Book ID
110854602
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
292 KB
Volume
30
Category
Article
ISSN
0140-7791

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📜 SIMILAR VOLUMES


d-Ribulose-1,5-bisphosphate carboxylase-
✍ G.H. Lorimer; M.R. Badger; T.J. Andrews 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 580 KB

Improved methods for the activation and assay of D-ribulose-1,5-bisphosphate carboxylase and oxygenase are described. The importance of fully activating the enzyme before starting either reaction is emphasized. The enzyme is activated by preincubation with 20 mM M&l, and 10 mM NaHCO,, pH 8.6. To avo