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Rapid purification of a prekallikrein from rat pancreas

โœ Scribed by Rebecca Matsas; David Proud; Kjell Nustad; Graham S. Bailey

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
543 KB
Volume
113
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A prekallikrein from rat pancreas was purified ISOO-fold with an overall yield of 20% using a rapid, simple procedure. DEAE-Sephadex A-50 chromatography permitted the separation of two prekallikrein components present in rat pancreatic homogenates; the major fraction was further purified by Sephadex G-100 gel filtration and immunoadsorption chromatography. The zymogen is a single-chain molecule with pl 4.35. Apparent M, values of 38,000 and 37,000 were determined by sodium dodecyl sulfate-polyacrylamide gradient electrophoresis and gel filtration, respectively.

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