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A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes

✍ Scribed by Elizabeth A. Shephard; Susan F. Pike; Brian R. Rabin; Ian R. Phillips

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
696 KB
Volume
129
Category
Article
ISSN
0003-2697

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✦ Synopsis

NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this single step procedure is as pure as that isolated by multicolumn procedures.

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