Rapid peptide bond formation on isolated 50S ribosomal subunits
β Scribed by Wohlgemuth, Ingo; Beringer, Malte; Rodnina, Marina V
- Book ID
- 110033078
- Publisher
- Nature Publishing Group
- Year
- 2006
- Tongue
- English
- Weight
- 152 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1469-221X
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π SIMILAR VOLUMES
The effect of spermine on the binding of AcPhe-tRNA to poly(U)-programmed ribosomes (step 1) and on the puromycin reaction (step 2) has been studied in a cell-free system, derived from E. coli. In the absence of ribosomal wash (FWR fraction) and at suboptimal concentration of Mg++ (6 mM), spermine s
50 S subunits of E. coli ribosomes catalyze the reaction of the 2'(3')-N-(formyl) methionine ester of adenosine 5'-phosphate and Phe-tRNA resulting in peptide bond synthesis. Cytidine 5'-phosphate stimulates this process on 50 S ribosomal subunits as well as on intact ribosomes. The obtained data sh