Rapid PCR-RFLP method for discrimination of imported and domestic mackerel
✍ Scribed by Futoshi Aranishi
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 160 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1053-6426
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A DNA-based method (PCR-RFLP) has been developed for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). The polymerase chain reaction (PCR) was used for ampli®cation of a 464 bp fragment of the mitochondrial cytochrome oxidase subunit II (COII) gene. Digest
## Abstract The polymerase chain reaction (PCR) is widely used to ascertain absolute or relative changes in the expression levels of specific genes as a function of cell type or in response to changes in environmental stimuli. Real‐time PCR is an advance which allows for the analysis of gene expres