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Rapid partial purification of S-adenosyl-L-methionine decarboxylase by affinity chromatography

✍ Scribed by Carol-Ann Manen; Diane H. Russell


Book ID
118936642
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
302 KB
Volume
14
Category
Article
ISSN
0024-3205

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Measurement of S-adenosyl-l-methionine l
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Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]adenosyl-L-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl-3H]adenosyl-L-methionine from [methyl-3H]methionine and other metabolites was achieved by stepwise elution with v