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Measurement of S-adenosyl-l-methionine levels by SP sephadex chromatography

✍ Scribed by Robert I. Glazer; Ann L. Peale

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 249 KB
Volume: 91
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90538-9

No coin nor oath required. For personal study only.

✦ Synopsis

Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]adenosyl-L-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl-3H]adenosyl-L-methionine from [methyl-3H]methionine and other metabolites was achieved by stepwise elution with varying concentrations of HCl.

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