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Rapid oligodeoxyribonucleotide synthesis by the “filtration” method

✍ Scribed by Bhabatosh Chaudhuri; Colin B. Reese; Krystyna Wȩcl̵awek


Book ID
107858364
Publisher
Elsevier Science
Year
1984
Tongue
French
Weight
258 KB
Volume
25
Category
Article
ISSN
0040-4039

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A method is described for the rapid analysis of DNA ligation products in the assembly of synthetic genes and gene fragments. The method is based on the simultaneous analysis of multiple ligation reactions where a single but different DNA oligomer is radiolabelled per ligation reaction. After each li