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Rapid methods for comparing the double-stranded RNA genome profiles of bluetongue virus

โœ Scribed by Squire, K.R.E.; Chuang, R.Y.; Osburn, B.I.; Knudson, D.L.; Doi, R.H.


Book ID
122893480
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
734 KB
Volume
8
Category
Article
ISSN
0378-1135

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A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" remo