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Rapid method for the separation and detection of tissue short-chain coenzyme A esters by reversed-phase high-performance liquid chromatography

✍ Scribed by Abraham Demoz; Alexis Garras; Daniel K. Asiedu; Bjørn Netteland; Rolf Kristian Berge


Publisher
Elsevier Science
Year
1995
Weight
291 KB
Volume
667
Category
Article
ISSN
0378-4347

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Extraction of tissue long-chain acyl-CoA
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Long-chain acyl-CoA esters were extracted from freeze-clamped livers of fed and fasted rats according to the method of Mancha et al. [M. Mancha, G. B. Stokes, and P. K. Stumpf (1975) Anal. Biochem. 68, 600-6081 and analyzed on a radially compressed Cis, 5 pm, reverse-phase column using a gradient sy