Rapid Isolation of High‐Quality Total RNA from Taxus and Ginkgo
✍ Scribed by Liao, Zhihua; Chen, Min; Guo, Liang; Gong, Yifu; Tang, Feng; Sun, Xiaofen; Tang, Kexuan
- Book ID
- 125489985
- Publisher
- Taylor and Francis Group
- Year
- 2004
- Tongue
- English
- Weight
- 114 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1082-6068
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A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr
The isolation of large quantities of good-quality RNA from human articular cartilage has been a long-standing problem for researchers working with human articular cartilage. In this paper we report a protocol which we have developed based on the Qiagen RNeasy procedure to produce high yields of puri