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Rapid isolation of glucose-6-phosphate dehydrogenase from human erythrocytes by combined affinity and anion-exchange chromatography for biochemical characterization of variants

โœ Scribed by Salvatore Pittalis; Franco Martinez di Montemuros; Dario Tavazzi; Gemino Fiorelli


Book ID
107997008
Publisher
Elsevier Science
Year
1992
Weight
429 KB
Volume
573
Category
Article
ISSN
0378-4347

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The isolation of glucose-6-phosphate dehydrogenase from outdated human erythrocytes (type AB+) utilizing NADP+ affinity chromatography and gel-permeation chromatography (Bio-Gel P-150) yields a fully active, homogeneous preparation. The procedure is faster and gives a higher yield than previous repo