✦ LIBER ✦

A rapid affinity chromatography procedure for the isolation of glucose-6-phosphate dehydrogenase from human erythrocytes

✍ Scribed by C.L. Craney; M.E. Goffredo

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 787 KB
Volume: 128
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90380-9

No coin nor oath required. For personal study only.

✦ Synopsis

The isolation of glucose-6-phosphate dehydrogenase from outdated human erythrocytes (type AB+) utilizing NADP+ affinity chromatography and gel-permeation chromatography (Bio-Gel P-150) yields a fully active, homogeneous preparation. The procedure is faster and gives a higher yield than previous reports.

📜 SIMILAR VOLUMES

Genetic variants of human erythrocyte gl

Genetic variants of human erythrocyte glucose 6-phosphate dehydrogenase: Automated procedure for characterization by column chromatography

✍ M. D'Urso; G. Battistuzzi; L. Luzzatto 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 398 KB

Glucose-6-phosphate dehydrogenase from L

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides is a reliable internal standard for radiation-inactivation studies of membranes in the frozen state

✍ J.Oliver McIntyre; Perry Churchill 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 872 KB

The target size of four soluble enzymes (beta-galactosidase, pyruvate kinase, alcohol dehydrogenase, and glucose-6-phosphate dehydrogenase) in the presence or absence of subcellular membrane fractions has been determined by the radiation-inactivation method using samples in the frozen state. For eac

Method for purification of large cyanoge

Method for purification of large cyanogen bromide peptides by carboxymethyl cellulose chromatography in 8 m urea: Application to the purification of cyanogen bromide peptides from staphylcoccal enterotoxin A, phosphoglycerate kinase, and glucose-6-phosphate dehydrogenase

✍ I-Yih Huang; Carol Ewing; Akira Yoshida 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 399 KB

A method for purification of large cyanogen bromide peptides from proteins by means of carboxymethyl cellulose chromatography in the presence of 8 M urea is described. Chromatography ofa number of large cyanogen bromide peptides which could not be separated by gel filtration showed that the resoluti

Application of covalent affinity chromat

Application of covalent affinity chromatography with thiol-disulphide interchange for determination of environmental exposure to heavy metals based on the quantitative isolation of Cd-thionein from human breast milk

✍ A. K. M. Kabzinski 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 147 KB 👁 2 views

The aim of this study was to test a new chromatographic method for the quantitative determination of Cd-thionein (Cd-Th) in human breast milk, in order to determine the level of exposure to heavy metals. Cd-thionein was isolated by covalent affinity chromatography with thiol-disulphide interchange,