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Application of covalent affinity chromatography with thiol-disulphide interchange for determination of environmental exposure to heavy metals based on the quantitative isolation of Cd-thionein from human breast milk

✍ Scribed by A. K. M. Kabzinski

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
147 KB
Volume
12
Category
Article
ISSN
0269-3879

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✦ Synopsis

The aim of this study was to test a new chromatographic method for the quantitative determination of Cd-thionein (Cd-Th) in human breast milk, in order to determine the level of exposure to heavy metals. Cd-thionein was isolated by covalent affinity chromatography with thiol-disulphide interchange, which is a modern separation technique of high affinity, good repeatability and reproducibility, allowing specific isolation of the thiolproteins. The fundamentals of indirect determination of the contents of metallothionein protein from human milk were worked out through estimation of the quantities of cadmium bound with Cd-thionein and adsorbed on covalent affinity chromatography gel during a separation process.

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✍ Andrzej K. M. Kabziński 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 221 KB 👁 2 views

Intoxication with heavy metals results in numerous poisonings and diseases. They disturb metabolism of the system, are the source of cancer, degeneration changes and others. As a result of kidney damage the urine of people exposed to heavy metals contains different low molecular weight proteins, oli