✦ LIBER ✦

RAPID IN SITU HARVESTING AND CYTOGENETIC ANALYSIS OF PERINATAL TISSUE SAMPLES

✍ Scribed by A. M. FISHER; A. E. COCKWELL; K. J. MOORE; N. M. GREGSON; P. L. CAMPBELL; C. M. CAMPBELL; A. HERBERT; J. C. K. BARBER; J. A. CROLLA

Book ID: 102650064
Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 526 KB
Volume: 16
Category: Article
ISSN: 0197-3851
DOI: 10.1002/(sici)1097-0223(199607)16:7<615::aid-pd920>3.0.co;2-9

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✦ Synopsis

Thirty perinatal solid tissue samples were used in a pilot study to test the efficacy of collagenase disaggregation onto coverslips, open system culture under low 0, conditions, pre-harvest incubation in bromodeoxyuridine (BrdU) and colcemid, and in situ automated harvesting. Following the success of the pilot study, the new method was applied to a further 126 consecutive diagnostic samples making a total of 156. The method reduced average tissue culture times from 17 to 8.7 days (range 2-17), improved success rates from 76 to 88 per cent, and simultaneously increased the resolution of cytogenetic analysis.

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