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Rapid hyperspectral fluorescence lifetime imaging

✍ Scribed by Pieter De Beule; Dylan M. Owen; Hugh B. Manning; Clifford B. Talbot; Jose Requejo-Isidro; Christopher Dunsby; James Mcginty; Richard K.P. Benninger; Daniel S. Elson; Ian Munro; M. John Lever; Praveen Anand; Mark A.A. Neil; Paul M.W. French

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
235 KB
Volume
70
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

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