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Multiphoton fluorescence lifetime imaging of human hair

✍ Scribed by Alexander Ehlers; Iris Riemann; Martin Stark; Karsten König

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
856 KB
Volume
70
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time‐resolved single photon counting and near‐infrared femtosecond laser pulse excitation was employed to analyze the various fluorescent hair components. Time‐resolved multiphoton imaging of intratissue pigments has the potential (i) to identify endogenous keratin and melanin, (ii) to obtain information on intrahair dye accumulation, (iii) to study bleaching effects, and (iv) to monitor the intratissue diffusion of pharmaceutical and cosmetical components along hair shafts. Microsc. Res. Tech. 2007. © 2006 Wiley‐Liss, Inc.

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