Multiphoton fluorescence lifetime imaging of 3D-stem cell spheroids during differentiation
✍ Scribed by Karsten König; Aisada Uchugonova; Erwin Gorjup
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 782 KB
- Volume
- 74
- Category
- Article
- ISSN
- 1059-910X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Long‐term high‐resolution multiphoton imaging of nonlabeled human salivary gland stem cell spheroids has been performed with submicron spatial resolution, 10.5‐nm spectral resolution, and picosecond temporal resolution. In particular, the two‐photon‐excited coenzyme NAD(P)H and flavins have been detected by time‐correlated single photon counting (TCSPC). Stem cells increased their autofluorescence lifetimes and decreased their total fluorescence intensity during the adipogenic‐differentiation process. In addition, the onset of the biosynthesis of lipid vacuoles was monitored over a period of several weeks in stem‐cell spheroids. Time‐resolved multiphoton autofluorescence imaging microscopes may become a promising tool for marker‐free stem‐cell characterization and cell sorting. Microsc. Res. Tech. 74:9‐17, 2011. © 2010 Wiley‐Liss, Inc.