Rapid fractionation of collagen chains a
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John F. Bateman; Thomas Mascara; Danny Chan; William G. Cole
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Article
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1986
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Elsevier Science
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English
β 940 KB
A strategy was developed, using a Pharmacia Fast Protein Liquid chromatography (FPLC) system, for the rapid preparation of the a-chains, cyanogen bromide peptides and tryptic peptides of type I collagen obtained from tissues and cultured fibroblasts. Collagen a-chains were prepared using a Cl8 PEP-