Rapid fractionation of collagen chains and peptides by high-performance liquid chromatography

✍ Scribed by John F. Bateman; Thomas Mascara; Danny Chan; William G. Cole

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 940 KB
• Volume: 154
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90534-8

✦ Synopsis

A strategy was developed, using a Pharmacia Fast Protein Liquid chromatography (FPLC) system, for the rapid preparation of the a-chains, cyanogen bromide peptides and tryptic peptides of type I collagen obtained from tissues and cultured fibroblasts.

Collagen a-chains were prepared using a Cl8 PEP-RPC reverse-phase column and volatile solvents. Preliminary Superose 6 gel permeation chromatography was used to separate the crosslinked @-and y-chains from the a-chains of tissue collagen samples. A Mono S cation-exchange column was used to resolve all ofthe major type I collagen cyanogen bromide peptides including the 01 I(I)CB7 and CB8 peptides, which have not been well resolved by previously published methods.

Collagen tryptic peptides were chromatographed on the PEP-RPC reverse-phase column.