Rapid exchange of histone H1.1 on chromatin in living human cells
β Scribed by Hendzel, Michael J.; Lever, Melody A.; Th'ng, John P. H.; Sun, Xuejun
- Book ID
- 109771939
- Publisher
- Nature Publishing Group
- Year
- 2000
- Tongue
- English
- Weight
- 238 KB
- Volume
- 408
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/35048603
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Nucleosomal subunits isolated from rabbit thymus nuclei in 0.04 M K2SO 4-0.02 M Tris, pH 7.4 were devoid of histone H1, while whole chromatin prepared in the same buffer contained the full complement of histone HI. The question is asked why histone H1 dissociates from the subunits but not from the h
## Abstract Understanding of cell regulation is limited by our inability to measure molecular binding rates for proteins within the structural context of living cells, and many systems biology models are hindered because they use values obtained with molecules binding in solution. Here, we present
H1 histones were purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis from human lung carcinoma (line DMS79), human hepatoblastoma (HepG2), human adult lung and human adult and fetal liver. The purified human H1 histones were analyzed for their amino acid composition and