Rapid automated derivatization of solid-
โ
Richard T. Pon; Shuyuan Yu
๐
Article
๐
1997
๐
Elsevier Science
๐
French
โ 252 KB
Very fast (4 sec) attachment of nucleosides to CPG supports, via succinyl or QDA linkers, is possible with either uronium (HBTU, HATU, HBPyU, HBPipU) or phospbonium (BOP, PyBOP ยฎ, Py-BroP, BroP) coupling reagents and automated derivatization of CPG and polystyrene supports just prior to oligonucleot