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Rapid determination of the 3-methylhistidine content of meat using 1-fluoro-2,4-dinitrobenzene

โœ Scribed by Poulter, N.H.; Lawrie, R.A.


Book ID
122722283
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
279 KB
Volume
4
Category
Article
ISSN
0309-1740

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Use of 1-fluoro-2,4-dinitrobenzene as a
โœ Tadashi Sawahata; Robert A. Neal ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 404 KB

The use of 1 -fluoro-2,4-dinitrobenzene (FDNB) as a probe for the presence of hydrodisulfide groups in proteins is described. The technique involves derivatimtion of the protein with FDNB and subsequent treatment with dithiothreitol (DTT). The 2,4dinitrothiophenol (DNTP) released from the PDNB-deri