Determination of unsaturated glycosamino
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Anna-Marja Säämänen; Markku Tammi
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Article
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1984
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Elsevier Science
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English
⚖ 446 KB
Chondroitin sulfate isomers and hyaluronate, digested with chondroitinase AC II and separated as unsaturated disaccharides by thin-layer chromatography on cellulose, were quantitated with scanning spectrophotometry using reflectance measurement at 232 nm. The sensitivity of the assay was increased b