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Rapid determination of cell volume density in mammalian cell suspensions

โœ Scribed by William R. Tolbert; Mary M. Hitt; Joseph Feder

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
350 KB
Volume
106
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Cell volume density was shown to be a useful growth parameter to monitor both single cell and aggregate suspension cultures. A method is described to quickly and easily measure this parameter using the length of a cell pellet in a conical hematocrit tube. The tubes were prepared by sealing the small end of disposable micropipet tips, and for seven cell lines growth within the range from 0.1 to 5 ml/liter was reproducible with an average -C 5.6% SD. Good agreement was obtained for population doubling times determined from cell volume density, DNA concentration, and, where possible, cell number density.

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